Figure 11. Imaging of UV-induced apoptosis in transgenic animals.

Transgenic mice were shaved and UVB-irradiated (b) or mock irradiated (a). The irradiated area is outlined as a red square. After irradiation, mice were injected with the fluorescent substrate DDAOG (9H-{1,3-dichloro-9,9-dimethylacridin-2-one-7-yl} β-D-galactopyranoside) and imaged using a Xenogen IVIS system. As compared with the control animals, the UVB-treated animals showed a significant fluorescent signal 24 hours after radiation. (c) Control animals (open bar) showed an approximately 10% change in fluorescence, whereas UV-irradiated animals (solid bar) had an 80% mean increase in fluorescence activity. Error bars represent ± SD, n = 5 animals per group. (From [48], with permission).