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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 2. Imaging of Akt kinase inhibiton in live animals.

Figure 2

A, Mice transplanted with D54 cells expressing the Akt reporter were treated with vehicle control (20% DMSO in PBS), API-2 (20 mg/kg or 40 mg/kg), or Perifosine (30 mg/kg). Representative images are shown of mice prior to treatment or during maximal luciferase signal upon treatment (Max), and post-treatment. B, Tumor-specific bioluminescence activity of D54 cells stably expressing BAR in mice, treated with either the vehicle control (20% DMSO in PBS) or API-2 (20 mg/kg or 40 mg/kg), was monitored at various times. Fold induction of signal intensity over pretreatment values was plotted as mean ± SEM for each of the groups. C, Bioluminescence activity in tumor-bearing mice before and after treatment with 30 mg/kg perifosine was plotted as fold induction over pretreatment values (± SEM) for each of the groups. (From [17], with permission).