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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 3. Membrane-targeted bioluminescent Akt reporter.

Figure 3

MyrPalm-BAR is generated by adding 10 amino acids from the N-terminus of Lyn kinase to BAR plasmid. The proposed basis of reporter activity for the MyrPalm-BAR reporter remains the same as that for BAR alone, which is described in Figure 1. This involves Akt-dependent phosphorylation of the Akt peptide domain that results in its interaction with the FHA2 domain. In this form (Akt-ON), the reporter has minimal bioluminescence activity (Light OFF). In the absence of Akt activity (Akt-OFF), association of the N-Luc and C-Luc domains restores bioluminescence activity (Light-ON).