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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 5. Bioluminescent imaging reporter for apoptosis.

Figure 5

Depicted is a recombinant reporter created by fusing residues 281 to 599 of the modified mouse estrogen receptor (ER) sequence to the N- and C-terminal ends of luciferase, respectively, yet separated by DEVD sequence on both ends. Caspase-3–dependent cleavage of the ER domains from the luciferase of the ER–DEVD–Luc–DEVD–ER reporter restores luciferase activity.