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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 6. Noninvasive imaging of caspase-3 utilizing a split-luciferase reporter strategy.

Figure 6

A, Schematic representation of the bioluminescent caspase-3 reporter (AN-Luc-BC-Luc). Apoptosis imaging reporter constitutes the split luciferase (N-Luc and C-Luc) domains fused to interacting peptides, pepA and pepB, with an intervening caspase-3 cleavage motif. B, Upon induction of apoptosis, the reporter molecule is proteolytically cleaved by caspase-3 at the DEVD motif. This cleavage enables interaction between pepANLuc and pepBCLuc, thus reconstituting luciferase activity.