Skip to main content
. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 7. Tetrameric LacZ–based reporter for monitoring cell death.

Figure 7

A, Recombinant beta-galactosidase–based apoptosis reporter was created by fusing ER at both N- and C-terminus of LacZ separated by DEVD sequence on both ends. B, Addition of the ER to both end of LacZ had lead to inhibitory effect on the activity of ER–DEVD–LacZ–DEVD–ER: It inhibited the tetramerization of the enzyme, which is critical for its activity, leading to increased signal-to-noise ratio.