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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 8. Strategy for imaging of apoptosis based on conditional expression of single chain antibody.

Figure 8

A, Constitutive imaging construct (CIC) was constructed from a single chain antibody, signal peptide, HA and myc tags, and a transmembrane domain. When expressed in cells, this fusion protein localizes to the cell surface. B, The inducible imaging construct (IIC) was CIC engineered to contain a Golgi retention signal, separated by caspase-3 recognition and cleavage sequence. The DEVD sequence was placed between the transmembrane domain and a Golgi retention signal to retain caspase-3 cleavage sequence in the cytoplasm (see inset). When expressed in cells, this chimeric protein localizes to Golgi bodies. Induction of apoptosis leads to caspase-3 activation and cleavage of the chimeric protein resulting in its translocation to cell surface.