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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 9. TGN protease imaging reporter.

Figure 9

A, Fusion protein consisting of three functional domains, a secreted alkaline phosphatase (SEAP), a Golgi protease–specific recognition and cleavage site, and a Golgi retention signal from BACE that retains the reporter within the TGN, was created. This reporter localizes to TGN until it is cleaved by a specific TGN protease, after which SEAP is secreted into the extracellular media. The protease-specific recognition and cleavage sequence is located within the lumen of the Golgi to respond to specific TGN proteases residing in this compartment of the Golgi bodies (see inset).