Table 2.

A comparison of the microscopy platforms tested for FISSEQ.

	Model	Pros	Cons	Uses
Wide-field epifluorescence	Nikon TE-2000	Fast imaging Simple set-up	Poor axial resolution Low S/N ratio Lower read depth	Thin cells & tissue sections Whole cell barcode labeling
Scanning confocal	Zeiss LSM 710 confocal Leica TCS SP5 Confocal	Good axial resolution Scanning zoom Flexible pixel density	Slow imaging	High resolution FISSEQ of a single region
Spinning disk confocal	Yokogawa CSU-W1	Fast imaging Good axial resolution	Fixed pixel density	All-purpose