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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Nat Protoc. 2015 Feb 12;10(3):442–458. doi: 10.1038/nprot.2014.191
Component Amount (µl) Final
DEPC-H2O 159
M-MuLV RT buffer, 10× 20 1×
dNTP, 25 mM 2 250 µM
Aminoallyl dUTP, 4 mM 2 40 µM
RT primer, 100 µM (/5Phos/TCTCGGGAACGCTGAAGANNNNNN) 5 2.5 µM
RNase Inhibitor (40 U µl−1) 2 0.4 U µl−1
M-MuLV reverse transcriptase (100 U µl−1) 10 5 U µl−1
Total 200