Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 8;12:16. doi: 10.1186/s12985-015-0248-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Raut et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

DENV-2 NS2b-NS3Pro enzyme assay, optimization and validation. (A) Kinetics of NS2b-NS3Pro action as a function of substrate concentration (at 5nM enzyme). (B) Rate of enzyme catalysis as a function of enzyme concentration (at 10 μM substrate). (C) Activity of the cloned NS2b-NS3Pro as function of aprotinin concentration (5nM enzyme, 10 μM substrate, 20 min incubation). Activity in the absence of aprotinin was taken as 100% (RFU = relative fluorescence units).