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. 2015 Jan 31;11:12. doi: 10.1186/s12917-015-0318-8

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© Monthoux et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Light and electron microscopy of affected (a&c) and unaffected (b&d) skin. Light microscopy of affected (a) and unaffected (b) skin: The deep dermis is markedly thinned (approximately 30% of dermal thickness of hind limb skin of unaffected 1-day-old control Warmblood foal (b)) and shows a reduced number of thin, irregular collagen bundles separated by clear spaces. The epidermis and adnexal structures appear normal. Subcutaneous tissue was detached from the overlying dermis and is missing on this slide. The size bars indicate 200 μm. Haematoxylin and eosin staining (HE), 4x. Electron microscopy of affected (c) and unaffected (d) skin: In the affected skin (c) (front limb) a mild randomness in orientation and variability of the cross-sections of the collagen fibrils are present. The size bar indicates 0.5 μm. The skin of the control (d) (hind limb from a 1-day-old control Warmblood foal) shows regular orientation of collagen fibrils with no visible variability of cross-sections. The size bar indicates 1 μm.