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. 2015 Feb 7;14:18. doi: 10.1186/s12933-015-0177-4

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© Shi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Liraglutide (LIRA) inhibited high glucose (HG)-induced migration in cultured vascular smooth muscle cells (VSMCs). (A) Transwell filters were stained with hematoxylin to visualize migrated cells (200× magnification). (B) Quantitation of migrated VSMCs in the Transwell migration assay; ^* p < 0.01 vs. control; ^# p < 0.01 vs. HG. (C) Confluent VSMCs were wounded and each scratch was imaged at 0 and 24 h after wounding. The wound gaps in each culture were measured to indicate the migration capacity of VSMCs. (D) Quantification of the wound area in the scratch assays; ^* p < 0.01 vs. control; ^# p < 0.05 vs. HG. The results are presented as mean ± standard deviation from three independent experiments. MG = mannitol, osmotic control.