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. Author manuscript; available in PMC: 2015 Oct 22.
Published in final edited form as: Sci Transl Med. 2014 Oct 22;6(259):259ra146. doi: 10.1126/scitranslmed.3009815

Fig. 1. In vitro uptake of 18F-FDS in bacterial pathogens and mammalian cell lines.

Fig. 1

(A) 18F-FDS uptake in E. coli (ATCC 25922), K. pneumoniae (ATCC 35657), and S. aureus (ATCC 29213) cultures incubated with 18F-FDS or 18F-FDG. Heat-killed bacteria were negative controls. P values compared to respective heat-killed controls were determined by two-tailed Student’s t test. (B) Competition of 18F-FDS uptake with increasing concentrations of unlabeled (free) sorbitol in E. coli (normalized to E. coli uptake without sorbitol). (C) Homology of E. coli (ATCC 25922) sorbitol-6-phosphate dehydrogenase gene, srlD, to other pathogenic bacteria in the UniProtKB database. (D) Uptake of 18F-FDS in ATCC reference Gram-negative and Gram-positive bacterial strains incubated for 120 min. (E) J774 (murine macrophage), WEHI 164 (murine fibroblast), U87MG (human glioblastoma), and NCI-H460 (human large cell lung carcinoma) cultures were incubated with 18F-FDS or 18F-FDG. Uptake was measured at 120 min and is shown in comparison to the reference E. coli. Data in (A), (B), (D), and (E) are means ± SEM (n = 6).