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. Author manuscript; available in PMC: 2015 Mar 10.
Published in final edited form as: Exp Cell Res. 2014 Jan 4;322(1):133–148. doi: 10.1016/j.yexcr.2013.12.021

Figure 3. The apical junctional complex is maintained as cells migrate into the CMZ.

Figure 3

Wounded epithelial cultures were imaged at D0 and D1 after injury following immunostaining for (A,C,F,I,K, N) ZO-1 and (B,D,G,J,L,O) N-cadherin. (E,H,M,P) ZO-1/N-cadherin overlays. Z-stacks were collected and shown as (A,B,I,J) orthogonal cuts or (C-H, K-P) single optical planes at the cells’ apical and mid-lateral domains. At D0 ZO-1 is largely restricted to the cells’ apical domains (A,C). N-cadherin is similarly localized but also extends basally along the cells’ lateral borders, although at diminishing concentrations (B,D,G). At D1 there was little change in the distribution of ZO-1 (I,K) but increased levels of N-cadherin junctions in the cells’ mid-lateral zones (J,O). A higher magnification image included as an inset in (L), short white arrow indicates N-cadherin puncta. Arrows indicate the direction of migration. Mag. Bar = 20 μm. Studies are representative of at least three independent experiments.