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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nat Commun. 2014 Mar 18;5:3494. doi: 10.1038/ncomms4494

Figure 3. Apoptosis-resistant BM does not alter intestinal crypt response to 15 Gy TBI.

Figure 3

WT and PUMA KO BMT recipients were exposed to 15 Gy TBI. (a) TUNEL IF in BMT mice at the indicated times. (× 200 magnification). Scale bar, 50 μm. (b) Quantification of TUNEL-positive cells in intestinal crypts of mice treated as in a. (c) BrdU incorporation in the transplanted mice at the indicated times. (× 100 magnification); scale bar, 100 μm. (d) Quantification of BrdU-positive cells in intestinal crypts of mice treated as in a. (e) BrdU staining labelled regenerated crypts in transplanted mice. Scale bar, 50 μm. (f) Quantification of regenerated crypts from e. (g) CD166 IF in intestinal crypts of transplanted mice. (× 400 magnification). Scale bar, 25 μm. (h) Quantification of CD166+ cells in CD166+ crypts at the indicated times. Values in b, d, f and h represent means + s.d. for n =3 mice per group.