. Author manuscript; available in PMC: 2015 Mar 18.

Published in final edited form as: Nat Commun. 2014 Mar 18;5:3494. doi: 10.1038/ncomms4494

Table 1.

Contribution of transplanted BM in the small intestine.

Donor	Genotype	Epithelial	CD45⁺ cells	CD105⁺ cells	α-SMA⁺ cells
Fraction of GFP ⁺ cells in villi
Whole BM	WT	1.5%	86.7%	80.2%	4.0%
Whole BM	PUMA KO	4.0%	79.6%	83.4%	3.8%
CD45⁺ BM	WT	1.8%	85.7%	89.8%	4.5%
CD45⁺ BM	PUMA KO	2.9%	76.6%	87.6%	8.5%
Fraction of GFP ⁺ cells in crypts
Whole BM	WT	2.9%	95.5%	74.2%	3.8%
Whole BM	PUMA KO	1.7%	91.1%	76.5%	2.0%
CD45⁺ BM	WT	0.0%	88.5%	83.8%	0.0%
CD45⁺ BM	PUMA KO	0.0%	83.8%	87.5%	0.0%

α-SMA, α-smooth muscle actin; BM, bone marrow; GFP, green fluorescent protein; WT, wild type.

Intestinal sections were double stained with a lineage-specific marker and GFP. GFP + donor cells were then counted within each cell compartment, and recorded as a percentage of the total cells within each specific lineage. At least 100 crypt-villus structures were counted for each determination, n = 3 mice per group.