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. Author manuscript; available in PMC: 2015 Feb 14.
Published in final edited form as: J Recept Signal Transduct Res. 2010 Apr;30(2):61–71. doi: 10.3109/10799890903563768

Figure 4. Role of MAPK kinase on MAPK activation (A), the synthesis of PGI2 (6-keto-PGF1α, B) and cAMP (C) by BK and IGF-1.

Figure 4

VSMC were stimulated with either BK (10−8M) or IGF-1 (10−8M) for 10 min in the presence and absence of MEK inhibitor PD98059 (20 μM). MAPK phosphorylation (p42mapk and p44mapk) were measured by immunoblot using anti-phosphotyrosine-MAPK antibodies (p-MAPK) and total MAPK was measured in the same immunoblot by stripping the membrane and re-immunoblotting with anti-total MAPK antibodies (t-MAPK). Release of 6-keto-PGF1α and cAMP into the media, were measured by RIA. Data are expressed as mean±SE and the bar graphs are representative of 6 separate experiments. * P<0.05 vs. control, †P<0.05 vs. BK, #P<0.05 vs. IGF-1.