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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Microvasc Res. 2013 Dec 24;92:50–55. doi: 10.1016/j.mvr.2013.12.005

Figure 2. High amplitude 18% CS is additive with oxidative stress in HMGB1 upregulation in HLMVEC.

Figure 2

Real-time quantitative polymerase chain reaction of HMGB1 using β-actin as a housekeeping gene. 100μM H2O2 increases HMGB1 mRNA expression in HLMVEC at 4 hours in all conditions (static, 5% cyclic stretch, 18% cyclic stretch) with ~13-fold increase in EC challenged with both H2O2 and 18% CS. 5% CS significantly attenuates the H2O2-induced increase in HMGB1 expression seen in static EC, as analyzed by the Wilcoxon Rank Sum Test (p<0.005). HMGB1 mRNA expression is normalized to HMGB1 expression in unstimulated, static EC. Two way ANOVA shows that H2O2 challenge significantly increases HMGB1 expression (p<0.05) in all conditions (static, 5% CS, and 18% CS), that 18% CS significantly increases HMGB1 expression compared to static conditions (p<0.05), and shows a significant interaction (p<0.05) between 18% CS and H2O2 in increasing HMGB1 expression (* indicates p < 0.05).