Figure 2.

Confirmation of the ATCC31749 crdR mutant by PCR amplification. (A, B) crdR was replaced without and with gentamicin resistance gene (Gm) in ATCC31749; (C) PCR amplification. C_M: 2Kb DNA ladder, C₁, C₃, C₅: template of genomic DNA of wild-type ATCC31749, while C₂, C₄, C₆: template of genomic DNA of ATCC31749ΔcrdR candidate were used respectively. C₂: amplification of the Gm gene; C₄: amplification of a fused fragment of the crdR upstream flanking region and the 5’-end of the Gm gene; C₆: amplification of the fused fragment of 3’-end of the Gm and the crdR downstream flanking region.