Table 1.
Plasmids
| Plasmid | GenBank accession no. | Pertinent features a,b | Source |
|---|---|---|---|
| pTn7xLuxG0 | KF532964 | Gmr; luxCDEBA operon transcribed from P PA4974 contained on DraIII fragment | This Study |
| pTn7oLuxG0 | KF532961 | Gmr; DraIII fragment containing P PA4974 replaced with DraIII fragment containing P ompA c | This Study |
| pTn7xLuxG3 | KF532965 | Kmr; pTn7xLuxG0 with BamHI and PstI sites deleted by partial digestion | This Study |
| pTn7xLuxK3d | KC332283 | Kmr; pTn7xLuxG3 with Gmr encoding aacC1 gene replaced with Kmr encoding nptII gene from pFKM4 | This Study |
| pTn7oLuxK3d | KC332281 | Kmr; pTn7xLuxK3 with DraIII fragment containing P PA4974 replaced with DraIII fragment containing B. pseudomallei P ompA | This Study |
| pTn7tLuxK3d | KC332282 | Kmr; pTn7xLuxK3 with DraIII fragment containing P PA4974 replaced with DraIII fragment containing B. pseudomallei P tolC | This Study |
| pTn5/7LuxK3d | KC332286 | Kmr; pTn7xLuxK3 with StuI-DraIII fragment containing P PA4974 replaced with StuI-DraIII fragment containing Tn5 transposase gene tnpA and flanking mosaic ends | This Study |
| pTn7oLuxG4 | KF532962 | Gmr; pTn7oLuxG0 with BamHI multiple cloning site fragment deleted | This Study |
| pTn7oLuxK4 | KC332284 | Kmr; pTn7oLuxG4 with Gmr encoding aacC1 gene replaced with Kmr encoding nptII gene from pFKM4 | This Study |
| pTn7oLuxT4 | KF532963 | Tpr; pTn7oLuxG4 with Gmr encoding aacC1 gene replaced with Tpr encoding dhfRII gene from pFTP2 | This Study |
| pTn5/7LuxG4 | KF813061 | Gmr; pTn7oLuxG4 with StuI-DraIII fragment containing P ompA replaced with StuI-DraIII fragment containing Tn5 transposase gene tnpA and flanking mosaic ends | This Study |
| pTn5/7LuxK4 | KF532957 | Kmr; pTn7oLuxK4 with StuI-DraIII fragment containing P ompA replaced with StuI-DraIII fragment containing Tn5 transposase gene tnpA and flanking mosaic ends | This Study |
| pTn5/7LuxT4 | KF813062 | Tpr; pTn7oLuxT4 with with StuI-DraIII fragment containing P ompA replaced with StuI-DraIII fragment containing Tn5 transposase gene tnpA and flanking mosaic ends | This Study |
| pTn5/7LuxK5 | KF532958 | Kmr; pTn5/7LuxK4 with tnpA gene transcribed from P S12 | This Study |
| pTn5/7LuxT5 | KF532959 | Tpr; pTn5/7LuxT4 with tnpA gene transcribed from P S12 | This Study |
| pTn5/7LuxT6 | KC332285 | Tpr; pTn5/7LuxT5 with attB1 inserted at DraIII site | This Study |
| pTn5/7LuxG6 | KC332289 | Gmr; pTn5/7LuxT6 with Tpr encoding dhfRII gene replaced with Gmr encoding aacC1 gene from pFGM1 | This Study |
| pTn5/7LuxK6 | KC332287 | Kmr; pTn5/7LuxT6 with Tpr encoding dhfRII gene replaced with Kmr encoding nptII gene from pFKM4 | This Study |
| pTn5/7LuxTc6 | KC332288 | Tcr; pTn5/7LuxT6 with Tpr encoding dhfRII gene replaced with Tcr encoding tetA gene from pFTC2 | This Study |
| pTn7P DH51-lux | N/A | Kmr; captured DH5α rbsC::Tn5/7LuxK3 insertion on mini-Tn7 delivery plasmid obtained by religation of chromosomal Acc65I fragment | This Study |
| pTn7P rbsLuxK4 | N/A | Kmr; E. coli rbs promoter (P rbs) cloned into pTn7LuxK4 | This Study |
| pTn7P H1ALuxK5 | N/A | Kmr; A. baumannii A1S_0945 gene promoter (P H1A) cloned into pTn7LuxK5 | This Study |
aAbbreviations: Ap, ampicillin; FRT, Flp recombinase target; Gm, gentamicin; Km, kanamycin; r, resistance/resistant; Tc, tetracycline; Tp, trimethoprim.
bSee supplemental methods for details of plasmid constructions.
cPromoters used are: P PA4974, P. aeruginosa gene PA4974 promoter; P ompA, B. pseudomallei ompA promoter; P tolC, B. pseudomallei tolC promoter; P S12, B. thailandensis ribosomal S12 gene promoter.
dPlasmids are missing a FRT site flanking the nptII gene.