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. 2015 Feb 6;15(1):20. doi: 10.1186/s12866-014-0338-8

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© Vela-Corcía et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transformation of P. xanthii conidia with the plasmid pCPXBteGFP. (A) Untransformed colonies growing normally on non-treated cotyledons. (B) Growth of P. xanthii transformants resistant to carbendazim. Conversely to hygromycin B, almost no appreciable tissue damage was observed on zucchini cotyledons. (C-D) EGFP analysis of transformants by CLSM analysis. (C) No fluorescence signal was observed in untransformed colonies. (D) In resistant transformants the EGFP fluorescence signal was observed within the peripheral hyphae; however, no fluorescence was observed in conidia or subjacent hyphae. No colonies were observed on cotyledons treated with carbendazim and inoculated with untransformed conidia. Pictures were taken 15 d after electroporation of conidia. Scale bars 100 μm.