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. 2015 Feb 5;16(1):46. doi: 10.1186/s12864-014-1195-4

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© Jakobsen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of pico-scale transcription factor libraries. (A) CEBPA profiles of a 80 kb region on chromosome 1, based on progressively lower amounts of input ChIP-DNA and bacterial carrier (CEBPA-1, 300 and 1700 pg; CEBPA-3, 100 and 1900 pg; CEBPA-4, 100 and 900 pg; CEBP-5, 100 and 400 pg; CEBPA-6, 50 and 450 pg). (B) Quantitative comparisons of mean read coverage of CEBPA-1 versus CEBPA-3 /-4/-5/-6. ChIP-seq profiles in CEBPA-1 peak regions defined by MACS2. Quantile normalized, 1/1 diagonal indicated by dashed line (r = pearson correlation, raw data). (C) SeqMiner cluster heatmaps showing signal intensities for profiles CEBPA-1 and CEBPA-6 at 10000 bp regions centered on peak summits. For B and C, peak set as in Figure 3B.