Table 2.
Microscopic examination of dermatological tissue sections with Dieterle or Warthin Starry silver nitrate stain and anti-Bb polyclonal immunostain
| Patient # | Dieterle | Bb-polyclonal immunostain | Filaments morphologically consistent with MD |
|---|---|---|---|
| 1 | Spirochetes observed | *Positive | Detected |
| 2 | Spirochetes observed | *Positive | Detected |
| 3 | Spirochetes observed | *Positive | Detected |
| 4 | Spirochetes observed | *Positive | Detected |
| 5 | Spirochetes not observed | **Positive | Detected |
| 8 | Spirochetes observed | Positive | Detected |
| 10 | Spirochetes observed | Positive | Detected |
| 11 | Spirochetes observed | Positive | Detected |
| 12 | Spirochetes observed | Positive | Detected |
| 13 | Spirochetes observed | Positive | Detected |
| 15 | Spirochetes observed | Positive | Detected |
| 16 | Spirochetes observed | Positive | Detected |
| 18 | Spirochetes observed | Positive | Detected |
| 19 | Spirochetes observed | Positive | Detected |
| 20 | Spirochetes observed | Positive | Detected |
| 22 | Spirochetes observed | Positive | Detected |
| 23 | Spirochetes observed | Positive | Detected |
| 24 | Spherules consistent with Bb morphological variants observed | Positive | Detected |
| 25 | Spirochetes observed | Positive | Detected |
Dieterle and or Warthin-Starry silver nitrate staining performed at Interscope Laboratories, Los Angeles, CA, and or McClain Laboratories, Smithtown, NY.
Bb polyclonal immunostaining performed at McClain Laboratories.
*Polyclonal Bb immunostaining performed at University of New Haven, West Haven, CT and McClain Laboratories.
**Sectioned specimen consisted of hair follicular bulbs with attached root sheaths rather than skin. Polyclonal Bb immunostaining performed at the University of New Haven.