Table 1.
HPAI H5 proposed clade 2.3.4.4 strains containing different N types
| Influenza A virus | Subtype | Accession |
|---|---|---|
| A/wild duck/Shandong/628/2011 | H5N1 | JX534565 |
| A/chicken/China/AH/2012 | H5N1 | KC631941 |
| A/muscovy duck/Vietnam/LBM631/2014 | H5N1 | AB979455 |
| A/muscovy duck/Vietnam/LBM635/2014 | H5N1 | AB979487 |
| A/muscovy duck/Vietnam/LBM636/2014 | H5N1 | AB979495 |
| A/duck/Vietnam/LBM638/2014 | H5N1 | AB979503 |
| A/duck/Eastern China/1111/2011 | H5N2 | JQ041401 |
| A/goose/Eastern China/1112/2011 | H5N2 | JQ041402 |
| A/duck/Jiangsu/m234/2012 | H5N2 | JX507355 |
| A/duck/Guangdong/wy11/2008 | H5N5 | CY091627 |
| A/duck/Guangdong/wy19/2008 | H5N5 | CY091635 |
| A/environment/Zhenjiang/C13/2013 | H5N6 | KJ938658 |
| A/duck/Guangdong/GD01/2014 | H5N6 | KJ754145 |
| A/duck/Lao/XBY004/2014 | H5N6 | KM496977 |
| A/duck/Jiangsu/k1203/2010 | H5N8 | JQ973694 |
| A/duck/Zhejiang/W24/2013 | H5N8 | KJ476669 |
| A/duck/Zhejiang/6D18/2013 | H5N8 | KJ476670 |
| A/breeder duck/Korea/Gochang1/2014 | H5N8 | KJ413834 |
| A/broiler duck/Korea/Buan2/2014 | H5N8 | KJ413842 |
| A/baikal teal/Korea/Donglim3/2014 | H5N8 | KJ413850 |
| A/chicken/Kumamoto/1-7/2014 | H5N8 | AB932556 |
The influenza A virus, segment 4 hemagglutinin (HA) gene sequences were identified by BLASTn analysis of HA sequence from A/duck/Laos/XBY004/2014(H5N6) (GenBank accession performed on 2nd September, 2014). The listed strains share 100% identity to the target region of probes D-720 PRB and D-724 PRB (MGB) of the proposed H5 clade 2.3.4.4 specific qRT-PCR assays. The H5 strains are sorted by N subtype N1, N2, N5, N6, and N8.