Figure 3.

Effect of Australian Aboriginal plant extracts at 100 μg/ml on basal and insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Cells were treated with individual extracts for 24 hours followed by incubation for 60 min in serum and glucose-free medium containing 80 μM 2-NBDG. Ethanol was used as a negative control, while rosiglitazone and insulin were used as positive controls. Cells received insulin only during 2-NBDG uptake. After incubation, fluorescence activity remaining in the cells was measured by a fluorescence microplate reader. Fluorescence activity in the absence of 2-NBDG was subtracted from all values. Data shown are mean ± SD of at least three independent experiments performed in triplicates. Significance against ethanol control (=100%): **p < 0.01, ***p < 0.001. Significance against ethanol + 100 nM insulin control: + p < 0.05, ++ p < 0.01, +++ p < 0.001.