Figure 4.

ELISA test. Titer of serum antibody is increased in mice by Escherichia coli -expressed recombinant EDIII3 protein formulated with Freund's adjuvant sera derived from mice which were immunized with PBS, as negative control group (green triangles), sera derived from mice which were vaccinated with EDIII3, as test group (red squares) and anti-dengue monoclonal antibody (instead of immune sera), as positive control (blue rhombus). These data demonstrated the potential of recombinant EDIII3 protein for inducing a high titer of specific antibody