Figure 6.

Cytokine profile of mice which were immunized with EDIII3. Splenocytes derived from immunized mice were restimulated with antigens thereafter the evaluation of cytokine release was done using commercial quantitative ELISA kits for IFN-γ and IL4. Splenocytes derived from mice which were immunized with adjuvant in combination with PBS and restimulated with EDIII3 antigen in vitro, were considered as negative control. The splenocytes derived from unimmunized mice were stimulated with ConA in vitro as a positive control for cytokines assay