Table 1.
Susceptibility of E. coli ATCC25922 transformed with the pTrcHis TOPO® TA vector carrying the cloned β-lactamase genes
| β-Lactamase/strain | MIC [mg/L] | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ceftazidime | Ceftazidime, BIC | Cefepime | Cefepime, BIC | Ertapenem | Ertapenem, BIC | Imipenem | Imipenem, BIC | Meropenem | Meropenem, BIC | Doripenem | Doripenem, BIC | |
| ATCC25922 | 0.125 | 0.125 | 0.064 | 0.032 | 0.016 | 0.032 | 0.125 | 0.064 | 0.016 | 0.016 | 0.016 | 0.016 |
| TEM-1 | 0.5 | 0.25 | 0.25 | 0.064 | 0.064 | 0.125 | 0.125 | 0.125 | 0.032 | 0.032 | 0.032 | 0.016 |
| DHA-1 | >32 | >32 | 0.25 | 0.125 | 0.064 | 0.25 | 0.125 | 0.064 | 0.016 | 0.064 | 0.032 | 0.016 |
| OXA-1 | 0.5 | 0.25 | 1 | 0.5 | 0.064 | 0.125 | 0.125 | 0.125 | 0.016 | 0.032 | 0.016 | 0.016 |
| OXA-9 | 0.25 | 0.25 | 0.25 | 0.064 | 0.032 | 0.125 | 0.125 | 0.125 | 0.032 | 0.032 | 0.032 | 0.016 |
| OXA-48 | 0.5 | 0.25 | 0.5 | 0.125 | 2 | 16 | 1 | 0.125 | 0.25 | 2 | 0.25 | 1 |
| KPC-2 | >32 | >32 | 2 | 1 | 0.5 | 2 | 0.5 | 0.25 | 0.25 | 0.5 | 0.125 | 0.5 |
Minimal inhibitory concentration determined by microdilution method with (BIC) and without sodium bicarbonate (50 mmol/L) and 1 mmol/L IPTG as an inducer. Significant changes are in bold
MIC minimal inhibitory concentration