Table 2.

Proteomic Profiling in Swine With Hibernating Myocardium With and Without. Revascularization^*

Protein Name	n	Average Ratio
		Hibernating LAD/sham	Revascularized LAD/sham
Citric Acid Cycle Proteins
Pyruvate dehydrogenase E1 α subunit	12	0.68†	0.74
Pyruvate dehydrogenase E1 β subunit	12	0.60†	0.77†‡
Dihydrolipoamide acetyltransferase (PDH E2)	12	0.74†	0.85†‡
Dihydrolipoamide dehydrogenase (PDH E3)	12	0.86	0.87
Dihydrolipoamide dehydrogenase (PDH E3)	12	0.77†	0.79
Aconitase	10	1.28†	1.05‡
Aconitase	12	1.06	1.05
Aconitase	12	0.86	0.98
Aconitase	9	0.95	0.81
Aconitase	11	1.00	1.18
NAD+ isocitrate dehydrogenase (α subunit)	12	0.69†	0.75†
NAD+ isocitrate dehydrogenase (α subunit)	12	0.72†	0.83†‡
Dihydrolipoamide succinyltransferase	12	0.87	0.96
Dihydrolipoamide succinyltransferase	11	0.97	1.02
Malate dehydrogenase (mitochondrial)	11	0.77	0.91‡
Malate dehydrogenase (mitochondrial)	9	0.85	0.91
Malate dehydrogenase (mitochondrial)	12	0.84	0.91
Electron Transport Chain and ATP Synthesis Proteins
NADH dehydrogenase (Complex I) 75 kDa subunit	12	0.86	0.84
NADH dehydrogenase (Complex I) 75 kDa subunit	11	0.72	0.76
NADH dehydrogenase (Complex I) 51 kDa subunit	12	0.82	0.89
NADH dehydrogenase (Complex I) 51 kDa subunit	12	0.67†	0.79†‡
NADH dehydrogenase (Complex I) 30 kDa subunit	10	0.61†	0.62†
NADH dehydrogenase (Complex I) 24 kDa subunit	11	0.78	0.88
Flavoprotein subunit of Complex II	12	0.58†	0.74‡
Cytochrome Bc1 core protein I (complex III)	12	0.69†	0.95‡
ATP synthase F1 α chain	7	0.87	1.00
ATP synthase F1 α chain	7	0.91	0.94
ATP synthase F1 α chain	12	0.86	0.89
ATP synthase F1 α chain	12	0.71†	0.81
ATP synthase F1 β chain	12	1.20	0.91‡
ATP synthase F1 β chain	12	0.84	0.77†
Fatty Acid Oxidation
Medium-chain acyl-CoA dehydrogenase (MCAD)	12	0.88	0.80
Long-chain acyl-CoA dehydrogenase (LCAD)	12	0.84	0.81‡
Other Mitochondrial Matrix Proteins
60 kDa heat shock protein, mitochondrial	12	0.95	0.94
Aspartate aminotransferase, mitochondrial	8	1.07	1.05
Creatine kinase, mitochondrial	6	1.01	0.96
Creatine kinase, mitochondrial	12	0.80	0.78
Isovaleryl-CoA dehydrogenase	12	0.78	0.82
Mitochondrial stress-70 protein (GRP 75)	12	0.92	0.98

Protein Name	n	Average Ratio
		Hibernating LAD/sham	Revascularized LAD/sham
NADP+ isocitrate dehydrogenase (47 kDa subunit)	11	0.58†	0.77‡
NADP+ isocitrate dehydrogenase (47 kDa subunit)	11	0.62	0.76‡
Succinyl-CoA 3-ketoacid-CoA transferase 1	12	0.69†	0.78†
Translation elongation factor EF-Tu	12	0.76†	0.84
Other Mitochondrial Membrane Proteins
Mitofilin	10	1.07	1.23
Mitofilin	12	0.78†	0.95
Prohibitin	3	0.83	0.95
Voltage-dependent anion channel 2 (VDAC2)	12	0.70†	0.96‡
Voltage-dependent anion channel 1 (VDAC1)	8	0.85	0.93
Voltage-dependent anion channel 1 (VDAC1)	12	0.87	0.97
Glycolytic Proteins
Fructose biphosphate aldolase A	7	0.61	0.78
Fructose biphosphate aldolase A	11	0.67	0.87‡
Triosephosphate isomerase	12	0.67†	0.73†
Triosephosphate isomerase	12	0.99	0.83†‡
GAPDH	11	1.58	1.19‡
GAPDH	12	1.27	0.93‡
GAPDH	12	0.96	0.77‡
GAPDH	8	1.05	0.87‡
Phosphoglycerate kinase 1	6	0.67†	0.90‡
Phosphoglycerate mutase 2 M isozyme	12	0.70†	0.71†
Enolase 3	12	0.94	0.90
α Enolase	11	0.98	1.09
β Enolase	12	0.84	0.86
Muscle specific phosphopyruvate hydratase (enolase)	12	0.81†	0.77†
Pyruvate kinase	12	0.66†	0.86
Anaerobic Metabolism Proteins
L-lactate dehydrogenase B chain	6	0.64†	0.68†
L-lactate dehydrogenase B chain	12	0.68†	0.89‡
Contractile Proteins
Actin, alpha-cardiac	12	0.83	0.89
Actin, alpha-cardiac	12	1.20	1.01‡
Actin, alpha-cardiac	3	0.80	0.71
Actin, alpha-cardiac	11	1.03	0.86
Actin, alpha-cardiac	12	0.65†	0.86‡
Actin, alpha-cardiac	11	0.45†	0.57†‡
Actin, alpha-cardiac	8	0.58†	0.78‡
Actin, alpha-cardiac	10	0.92	0.77†‡
Myosin heavy chain α (cardiac)	11	0.99	0.92
Myosin heavy chain α (cardiac)	10	0.66†	1.29
Myosin heavy chain β	8	0.69	0.95
Myosin heavy chain β	6	0.77	0.88
Myosin heavy chain β	6	0.92	1.08
Myosin heavy chain β	3	1.20	0.84

Protein Name	n	Average Ratio
		Hibernating LAD/sham	Revascularized LAD/sham
Myosin heavy chain β	12	0.94	0.95
Myosin heavy chain β	9	0.87	1.36
Myosin heavy chain β	4	1.46	1.11
Myosin heavy chain β	3	1.14	1.05
Myosin heavy chain β	9	1.23	1.07
Myosin light chain 3	11	0.65†	0.71†
Myosin light chain 3	11	0.60†	0.67†
Myosin light chain 3	11	0.83†	0.74†
Troponin T, cardiac	6	0.69	0.86‡
Troponin T, cardiac	12	0.70†	0.74†
Troponin T (cardiac isoform type 2)	12	0.67†	0.71†
Troponin T, cardiac	12	0.73†	0.60*‡
Troponin T cardiac	12	0.74†	0.64*‡
Tropomysin α chain	11	0.82	0.81
Tropomysin β chain	12	0.61†	0.63†
Structural/Cytoskeletal Proteins
Actin-interacting protein	12	0.79	0.97‡
Desmin	12	1.23	1.21
Desmin	11	1.14	0.87‡
Desmin	12	1.34†	0.90‡
Desmin	10	0.85	1.44
Desmin	12	1.37†	0.93‡
Gelsolin precursor	11	1.42	1.21
Vimentin	12	1.54†	1.10‡
Vinculin	12	1.14	1.09
Stress Proteins
αB crystallin	12	1.50†	0.90‡
αB crystallin	12	0.90	0.70†‡
Annexin 2	5	1.24	1.29
Annexin 2	12	1.05	1.04
GRP78	12	1.29	0.99‡
GRP78	12	1.22†	1.17
HSP70 (70 Kda protein 1)	12	1.11	0.99‡
HSP70 (70 Kda protein 1)	12	0.83	0.96‡
HSP60	12	0.95	0.94
HSP27	12	1.39	0.99
HSP27	12	1.07	0.91
HSP27	4	1.07	0.85
HSP20 β-6	12	1.69†	0.73†
T-complex protein 1 (chaperonin)	12	0.88	0.87
Antioxidant Proteins
Peroxiredoxin-2	12	0.90	0.87
Peroxiredoxin-6	12	0.73†	0.75†
Other Cytoplasmic Proteins
Aconitase 1 (Fe regulatory protein 1)	11	0.97	1.06

Protein Name	n	Average Ratio
		Hibernating LAD/sham	Revascularized LAD/sham
Aminoacylase-1	5	0.55†	0.68‡
Aspartate aminotransferase (cytoplasmic)	12	0.52†	0.64†
Creatine kinase, M chain (cytoplasmic)	11	0.84	0.69†‡
Creatine kinase, M chain (cytoplasmic)	12	0.73†	0.71†
Creatine kinase, M chain (cytoplasmic)	12	0.56†	0.65†
Cytosolic aminopeptidase	12	1.00	0.97
Dihydropyrimidinase-related protein 2 (DRP-2)	12	0.87†	0.83†
Malate dehydrogenase (cytoplasmic)	12	0.64†	0.69†
Myoglobin	8	0.62	0.61
Myoglobin	10	0.43†	0.36†
Transitional endoplasmic reticulum ATPase	11	0.99	1.04
Endoplasmic Reticulum Proteins
Protein disulfide isomerase A3	12	1.04	1.06

^*Bolded and italicized proteins are depicted in Figure 5.

^†p < 0.05 vs. sham

^‡p < 0.05 vs. hibernating