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. 2015 Feb 12;160(4):673–685. doi: 10.1016/j.cell.2015.01.008

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S5 — Characterization of Motile caRhoA-Expressing Cells In Vivo, Related to Figure 7

(A) Fluorescence time-lapse images of a single polarized stable-bleb cell transplanted from a transgenic Tg(actβ1:lifeact-GFP) donor with mosaic expression of caRhoA plus GPI-RFP into a wild-type host.

(B) Localization of Myl12.1-eGFP (myosin II) in a single polarized progenitor cell transplanted from a transgenic Tg(actβ1:myl12.1eGFP) donor with mosaic expression of caRhoA plus GPI-RFP into a wild-type host (left) with kymograph data along cell margin (right).

(C) Quantification of Myl12.1-eGFP (myosin II) localization in polarized stable-bleb cells along normalized cell length in vivo (n = 4 cells).

(D) Fluorescence time-lapse images of a single polarized stable-bleb cell transplanted from a transgenic Tg(actβ1:myl12.1eGFP) donor with mosaic expression of a constitutively active version of rhoA (caRhoA) plus GPI-RFP plus e-Cadherin morpholinos (MO) into a wild-type host.

(E) Exemplary 2D projected in vivo tracks of polarized motile cells expressing either caRhoA (blue) or caRhoA plus e-Cadherin MO (caRhoA+Ecad-MO, red) obtained from transplantation experiments as described in (D).

(F) Quantification of average single cell net displacement over time for polarized motile cells expressing either caRhoA (blue) or caRhoA plus e-Cadherin MO (caRhoA+Ecad-MO, red). All scale bars, 20 μm.