Table 1.
Results of real-time quantitative PCR to detect various viruses in the 39 patients with GBM and in positive control samples
| Viral DNA copy number* | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Case no. | Age | Sex | HCMV | MCPyV | HPyV6 | HPyV7 | HPyV9 | EBV | HHV8 | HPV | Cell number | ||||
| gB | IE | PCR** | Type 16 | Type 18 | PCR*** | ||||||||||
| 1 | 60 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 32 |
| 2 | 58 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 676 |
| 3 | 51 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | type 16 | 417 |
| 4 | 74 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 1280 |
| 5 | 72 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0.13 | 0 | type 16 | 21 |
| 6 | 68 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 135 |
| 7 | 49 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 770 |
| 8 | 71 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | type 16 | 118 |
| 9 | 60 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 370 |
| 10 | 42 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 34 |
| 11 | 42 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 221 |
| 12 | 82 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 1236 |
| 13 | 40 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 48 |
| 14 | 77 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 301 |
| 15 | 62 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 454 |
| 16 | 75 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 138 |
| 17 | 73 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 26 |
| 18 | 43 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 157 |
| 19 | 50 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 133 |
| 20 | 46 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 5 |
| 21 | 82 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 114 |
| 22 | 4 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 954 |
| 23 | 46 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 533 |
| 24 | 70 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 590 |
| 25 | 65 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 33 |
| 26 | 59 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | type 16 | 400 |
| 27 | 72 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 147 |
| 28 | 77 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 272 |
| 29 | 65 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.05 | type 18 | 50 |
| 30 | 72 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 214 |
| 31 | 77 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | type 16 | 388 |
| 32 | 51 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 2637 |
| 33 | 77 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 5269 |
| 34 | 80 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 7022 |
| 35 | 81 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 3510 |
| 36 | 63 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 3205 |
| 37 | 70 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | type 16 | 7315 |
| 38 | 70 | M | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ND | 705 |
| 39 | 88 | F | 0 | 0 | ND | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.18 | type 18 | 170 |
| HCMV-P1a | 827.37 | 2.01 | 17 | ||||||||||||
| HCMV-P2 | 671.98 | 270.96 | 123 | ||||||||||||
| HCMV-P3 | 3955.12 | 3748.20 | 10 | ||||||||||||
| MCPyV-P1 | 25879.50 | 188 | |||||||||||||
| MCPyV-P2 | 28.95 | 275 | |||||||||||||
| HPyV6-P1 | 107.60 | 92 | |||||||||||||
| HPyV6-P2 | 2.12 | 10 | |||||||||||||
| HPyV6-P3 | 0.20 | 254 | |||||||||||||
| HPyV7-P1 | 0.12 | 26 | |||||||||||||
| HPyV7-P2 | 0.45 | 10 | |||||||||||||
| HPyV7-P3 | 0.45 | 672 | |||||||||||||
| EBV-P1 | 61315.24 | 2316 | |||||||||||||
| EBV-P2 | 47466.54 | 2520 | |||||||||||||
| HHV8-P1 | 49621.12 | 1624 | |||||||||||||
| HPV16-P1 | 3.66 | 32 | |||||||||||||
| HPV16-P2 | 235.94 | 228 | |||||||||||||
| HPV16-P3 | 491.46 | 348 | |||||||||||||
| HPV16-P4 | 46.06 | 522 | |||||||||||||
| HPV18-P1 | 0.11 | 496 | |||||||||||||
| HPV18-P2 | 82.98 | 572 | |||||||||||||
| HPV18-P3 | 4.58 | 1191 | |||||||||||||
| HPV18-P4 | 0.04 | 10 | |||||||||||||
*Viral DNA copy number is shown as per reaction.
**Results of nested PCR are also shown.
***Results of nested PCR and the HPV type are shown.
aPositive controls are as follows: HCMV-P1, HCMV+ pancreatitis (FFPE); HCMV-P2, HCMV+ enteritis (FFPE); HCMV-P3, HCMV+ pneumonia; MCPyV-P1, MCPyV+ Merkel cell carcinoma (FFPE); MCPyV-P2, MCPyV+ cutaneous squamous cell carcinoma (FFPE); HPyV6-P1, HPyV6+ basal cell carcinoma (FFPE); HPyV6-P2, HPyV6+ melanoma (FFPE); HPyV6-P3, HPyV6+ cutaneous squamous cell carcinoma (FFPE); HPyV7-P1, HPyV7+ basal cell carcinoma (FFPE); HPyV7-P2, HPyV7+ melanoma (FFPE); HPyV7-P3, HPyV7+ cutaneous squamous cell carcinoma (FFPE); EBV-P1 and -P2, EBV+ lymphoblastoid cells (cell lines); HHV8-P1, HHV8+ KS-1 (cell line); HPV16-P1 and -P2, HPV16+ cervical cancer (FFPE); HPV16-P3 and –P4, HPV16+ oropharyngeal cancer (FFPE); HPV18-P1 and –P2, HPV18+ cervical cancer (FFPE); HPV18-P3 and –P4, HPV18+ oropharyngeal cancer (FFPE).
F: female; M: male; ND: not detected.