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. 2015 Jan 27;10:3. doi: 10.1186/1750-9378-10-3

Table 1.

Results of real-time quantitative PCR to detect various viruses in the 39 patients with GBM and in positive control samples

Viral DNA copy number*
Case no. Age Sex HCMV MCPyV HPyV6 HPyV7 HPyV9 EBV HHV8 HPV Cell number
gB IE PCR** Type 16 Type 18 PCR***
1 60 F 0 0 ND 0 0 0 0 0 0 0 0 ND 32
2 58 F 0 0 ND 0 0 0 0 0 0 0 0 ND 676
3 51 M 0 0 ND 0 0 0 0 0 0 0 0 type 16 417
4 74 F 0 0 ND 0 0 0 0 0 0 0 0 ND 1280
5 72 M 0 0 ND 0 0 0 0 0 0 0.13 0 type 16 21
6 68 F 0 0 ND 0 0 0 0 0 0 0 0 ND 135
7 49 M 0 0 ND 0 0 0 0 0 0 0 0 ND 770
8 71 M 0 0 ND 0 0 0 0 0 0 0 0 type 16 118
9 60 M 0 0 ND 0 0 0 0 0 0 0 0 ND 370
10 42 M 0 0 ND 0 0 0 0 0 0 0 0 ND 34
11 42 M 0 0 ND 0 0 0 0 0 0 0 0 ND 221
12 82 F 0 0 ND 0 0 0 0 0 0 0 0 ND 1236
13 40 M 0 0 ND 0 0 0 0 0 0 0 0 ND 48
14 77 M 0 0 ND 0 0 0 0 0 0 0 0 ND 301
15 62 F 0 0 ND 0 0 0 0 0 0 0 0 ND 454
16 75 M 0 0 ND 0 0 0 0 0 0 0 0 ND 138
17 73 M 0 0 ND 0 0 0 0 0 0 0 0 ND 26
18 43 F 0 0 ND 0 0 0 0 0 0 0 0 ND 157
19 50 F 0 0 ND 0 0 0 0 0 0 0 0 ND 133
20 46 M 0 0 ND 0 0 0 0 0 0 0 0 ND 5
21 82 M 0 0 ND 0 0 0 0 0 0 0 0 ND 114
22 4 F 0 0 ND 0 0 0 0 0 0 0 0 ND 954
23 46 M 0 0 ND 0 0 0 0 0 0 0 0 ND 533
24 70 F 0 0 ND 0 0 0 0 0 0 0 0 ND 590
25 65 F 0 0 ND 0 0 0 0 0 0 0 0 ND 33
26 59 F 0 0 ND 0 0 0 0 0 0 0 0 type 16 400
27 72 M 0 0 ND 0 0 0 0 0 0 0 0 ND 147
28 77 M 0 0 ND 0 0 0 0 0 0 0 0 ND 272
29 65 F 0 0 ND 0 0 0 0 0 0 0 0.05 type 18 50
30 72 M 0 0 ND 0 0 0 0 0 0 0 0 ND 214
31 77 M 0 0 ND 0 0 0 0 0 0 0 0 type 16 388
32 51 F 0 0 ND 0 0 0 0 0 0 0 0 ND 2637
33 77 F 0 0 ND 0 0 0 0 0 0 0 0 ND 5269
34 80 M 0 0 ND 0 0 0 0 0 0 0 0 ND 7022
35 81 M 0 0 ND 0 0 0 0 0 0 0 0 ND 3510
36 63 F 0 0 ND 0 0 0 0 0 0 0 0 ND 3205
37 70 M 0 0 ND 0 0 0 0 0 0 0 0 type 16 7315
38 70 M 0 0 ND 0 0 0 0 0 0 0 0 ND 705
39 88 F 0 0 ND 0 0 0 0 0 0 0 0.18 type 18 170
HCMV-P1a 827.37 2.01 17
HCMV-P2 671.98 270.96 123
HCMV-P3 3955.12 3748.20 10
MCPyV-P1 25879.50 188
MCPyV-P2 28.95 275
HPyV6-P1 107.60 92
HPyV6-P2 2.12 10
HPyV6-P3 0.20 254
HPyV7-P1 0.12 26
HPyV7-P2 0.45 10
HPyV7-P3 0.45 672
EBV-P1 61315.24 2316
EBV-P2 47466.54 2520
HHV8-P1 49621.12 1624
HPV16-P1 3.66 32
HPV16-P2 235.94 228
HPV16-P3 491.46 348
HPV16-P4 46.06 522
HPV18-P1 0.11 496
HPV18-P2 82.98 572
HPV18-P3 4.58 1191
HPV18-P4 0.04 10

*Viral DNA copy number is shown as per reaction.

**Results of nested PCR are also shown.

***Results of nested PCR and the HPV type are shown.

aPositive controls are as follows: HCMV-P1, HCMV+ pancreatitis (FFPE); HCMV-P2, HCMV+ enteritis (FFPE); HCMV-P3, HCMV+ pneumonia; MCPyV-P1, MCPyV+ Merkel cell carcinoma (FFPE); MCPyV-P2, MCPyV+ cutaneous squamous cell carcinoma (FFPE); HPyV6-P1, HPyV6+ basal cell carcinoma (FFPE); HPyV6-P2, HPyV6+ melanoma (FFPE); HPyV6-P3, HPyV6+ cutaneous squamous cell carcinoma (FFPE); HPyV7-P1, HPyV7+ basal cell carcinoma (FFPE); HPyV7-P2, HPyV7+ melanoma (FFPE); HPyV7-P3, HPyV7+ cutaneous squamous cell carcinoma (FFPE); EBV-P1 and -P2, EBV+ lymphoblastoid cells (cell lines); HHV8-P1, HHV8+ KS-1 (cell line); HPV16-P1 and -P2, HPV16+ cervical cancer (FFPE); HPV16-P3 and –P4, HPV16+ oropharyngeal cancer (FFPE); HPV18-P1 and –P2, HPV18+ cervical cancer (FFPE); HPV18-P3 and –P4, HPV18+ oropharyngeal cancer (FFPE).

F: female; M: male; ND: not detected.