Descriptions of cyclone and wet-walled cyclone spore traps

Sampler	Description	Ref	Flow rate (L min-1)	Collection Efficiency d50 (µm)*	Sample period	Compatible with analysis method
						Culture	Microscope	Biosensor/molecular
Burkard cyclone/multivial cyclone http://www.burkard.co.uk/autocycsamp.htm	Dry cyclone sampling into 1.5 mL tube into which particles settle because of a change in direction of vortexing air as it leaves via its central axis	González-Parrado et al. (2014) Bock & Cotty (2003)	16.6	20+		Yes (suspension and plating needed)	Yes (suspension in liquid needed)	Yes
NIOSH one-stage BC 112 sampler and NIOSH 2-stage BC 212 sampler	Dry cyclone or two-stage cyclone with terminal filter cassette (Various filters are compatible)	(1-stage): Chen et al. (2004).“. (2-stage) Lindsley et al. (2006)	4 (1-stage) 3.5 (2-stage)	1.5 (1-stage) 1.8 (2nd of-stage) and 1 (1st of 2-stage)	Variable	Yes	Needs processing	Yes
Coriolis®μ and Coriolis Recon Air Samplers http://www.coriolis-airsampler.com	Wet-wall cyclone	Carvalho et al. (2008)	100-300 (μ) 600 (Recon)	<1	1-10 minutes (μ) Up to 6h with long term facility option	Yes	Yes	Yes
Biral Aerosol Particle Collector http://www.biral.com/apc	Wet-wall cyclone		‘high’	1	‘Many hours’ depending on replenishment of circulating fluid	Yes	Yes	Yes
SKC biosampler http://www.skcltd.com/index.php/air-sampling-pumps/9-uncategorised/208-biosampler	Wet-wall cyclone	Willeke et al. (1998)	12.6	<0.2	<8h	Yes	Yes	Yes
Biotrace Biological Detection System (BBDS)	Wet-walled cyclone		750	<1	Continuous	A continuous flow ATP detector utilises ATP bioluminescence, for rapid detection of viable microbes in air
CIP 10-M Personal microbe sampler	gentle impaction onto a liquid-filled rotating cup	Görner et al. (2005); Nieguitsila et al. (2011)	10	1.8	1–200 min	Yes	Yes	Yes

^*d50 is the aerodynamic diameter or size of particles above which 50% or more are collected and below which, less than 50% are collected.