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. 2015 Feb 6;16(1):60. doi: 10.1186/s12864-015-1287-9

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© Fang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative real-time PCR validation of the differentially expressed genes. The relative expression of a candidate gene was normalized against RpL3. For the up-regulated DEGs in the domestic silkworms, the fold-change of each gene was calculated by dividing the relative expression level in the W_AKSQ. For the up-regulated DEGs in the wild silkworms, the fold-change of each gene was calculated by dividing the relative expression level in the D_CH. The data are the average ± standard error of three independent replicated qPCR experiments. An absolute value of fold-change ≥ 2 and one-way analysis of variance analysis (P-value < 0.005) were used to estimate the significance of gene expression changes. Significant differential expressions of genes between any two silkworms were marked by a star.