. 2015 Feb 4;8:72. doi: 10.1186/s13071-015-0666-z

Table 1.

Detail of the biopsies taken from mummy A74 and submitted to paleomolecular investigations, the primers used and the results obtained

Type of biopsies	Primers used	Results
Fragment of tissues adhering to the 10^th rib (Figure 1E)	13A: 5′ GGGGAGGGGCGTTCTGCGAA 3′	+
	13B: 5′ SSSCCMCTATWTTACACCAACCCC 3′ [36]	+
	13A: 5′ GTGGGGGAGGGGCGTTCT 3′	-
	13B: 5′ ATTTTACACCAACCCCCAG 3′ [37]	-
Fragments of tissues from abdominal region (Figure 1D1)	13A: 5′ GGGGAGGGGCGTTCTGCGAA 3′	+
	13B: 5′ SSSCCMCTATWTTACACCAACCCC 3′ [36]	+
	13A: 5′ GTGGGGGAGGGGCGTTCT 3′	+
	13B: 5′ ATTTTACACCAACCCCCAG 3′ [37]	+
Fragment of tissues from abdominal cavity (Figure 1D2)	13A: 5′ GGGGAGGGGCGTTCTGCGAA 3′	+
	13B: 5′ SSSCCMCTATWTTACACCAACCCC 3′ [36]*	+
	13A: 5′ GTGGGGGAGGGGCGTTCT 3′	-
	13B: 5′ ATTTTACACCAACCCCCAG 3′ [37]	-
Cortical bone from the 10^th rib (Figure 2A)	13A: 5′ GGGGAGGGGCGTTCTGCGAA 3′	-
Cortical bone from the 10^th rib (Figure 2A)	13B: 5′ SSSCCMCTATWTTACACCAACCCC 3′ [36]**	-
Bone marrow from the 10^th rib (Figure 2B)	13A: 5′ GGGGAGGGGCGTTCTGCGAA 3′	+
Bone marrow from the 10^th rib (Figure 2B)	13B: 5′ SSSCCMCTATWTTACACCAACCCC 3′ [36]**	+

* = Sample positive for Leishmania tarentolae in the first set of by using Rogers et al. [37] primers.

** = Samples from cortical bone and bone marrow analysed in a second round of analyses by using Degrave et al. [36] primers. With Degrave et al. (1994) primers, a more intense amplification of the PCR products was observed.