Table 1.

Population genetic parameters for 448 salamander larvae collected in Lombardy (Northern Italy): number of genotyped individuals (N), number of different alleles (Na), number of effective alleles (Ne), allelic range (AR), observed (Ho) and expected heterozygosity (He), fixation index (F_IS). Hardy–Weinberg Equilibrium test: degree of freedom (Df), chi-squared value (Chi-sq), P-value (P), significance after Holm–Bonferroni correction (HB sig).

Locus	Population genetic parameters							HWE test
	N	Na	Ne	AR	Ho	He	F IS	Df	Chi-sq	P	HB sig
All loci	446.06 (1.28)	9.94 (1.26)	2.79 (0.29)	–	0.546 (0.042)	0.579 (0.047)	0.048 (0.013)	32	Inf.	≪0.001	sig
SalE2	448	11	2.32	216–262	0.560	0.569	0.015	55	34.41	0.987	ns
Sal3	448	11	2.49	181–327	0.569	0.598	0.048	55	54.26	0.503	ns
SalE5	448	5	1.03	180–190	0.027	0.027	−0.011	10	0.08	1.000	ns
SalE6	448	5	2.34	277–297	0.527	0.572	0.079	10	6.23	0.795	ns
SalE7	448	11	2.75	184–232	0.643	0.636	−0.010	55	33.04	0.992	ns
SalE8	446	10	4.26	143–181	0.731	0.765	0.045	45	64.92	0.027	ns
SalE11	448	6	2.21	238–258	0.513	0.548	0.064	15	12.95	0.606	ns
SalE12	441	16	4.42	223–307	0.621	0.774	0.197	120	740.25	≪0.001	sig
SalE14	448	6	2.19	237–257	0.527	0.543	0.030	15	5.86	0.982	ns
Sal23	428	9	2.46	282–320	0.549	0.594	0.076	36	45.60	0.131	ns
SST-A6-I	448	6	1.75	207–231	0.420	0.428	0.020	15	5.05	0.992	ns
SST-A6-II	448	8	2.34	193–221	0.578	0.573	−0.010	28	16.75	0.953	ns
SST-B11	447	24	5.19	149–263	0.763	0.807	0.055	276	434.29	≪0.001	sig
SST-C2	447	13	3.06	194–246	0.620	0.673	0.079	78	105.81	0.020	ns
SST-C3	448	5	1.67	207–227	0.400	0.400	0.001	10	5.72	0.838	ns
SST-E11	448	13	4.13	233–311	0.690	0.758	0.090	78	96.21	0.079	ns

sig, significant; ns, not significant.