Table 4.
Population genetic parameters (with SE in parentheses) for the (a) two clusters of the PEF group and (b) four clusters of the WF group: number of genotyped individuals (N), number of different alleles (Na), number of effective alleles (Ne), allelic range (AR), observed (Ho) and expected heterozygosity (He), and fixation index (FIS). Hardy–Weinberg Equilibrium test: degree of freedom (Df), chi-squared value (Chi-sq), P-value (p), significance after Holm–Bonferroni correction (HB sig).
| Population genetic parameters | HWE test | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cluster | Locus | N | Na | Ne | Ho | He | F IS | DF | Chi-sq | P | HB sig |
| (a) | |||||||||||
| 1 | All loci | 278.9 (0.08) | 7.69 (0.91) | 2.51 (0.27) | 0.525 (0.049) | 0.542 (0.053) | 0.023 (0.011) | 26 | 34.88 | 0.114 | ns |
| SalE2 | 279 | 10 | 2.41 | 0.577 | 0.584 | 0.013 | 45 | 49.43 | 0.301 | ns | |
| Sal3 | 279 | 9 | 2.36 | 0.538 | 0.576 | 0.066 | 36 | 46.72 | 0.109 | ns | |
| SalE5 | 279 | 5 | 1.04 | 0.043 | 0.042 | −0.017 | 10 | 0.13 | 1.000 | ns | |
| SalE6 | 279 | 4 | 2.31 | 0.538 | 0.568 | 0.053 | 6 | 4.88 | 0.559 | ns | |
| SalE7 | 279 | 11 | 2.86 | 0.645 | 0.651 | 0.009 | 55 | 26.16 | 1.000 | ns | |
| SalE8 | 278 | 8 | 4.00 | 0.723 | 0.750 | 0.036 | 28 | 37.93 | 0.100 | ns | |
| SalE11 | 279 | 6 | 2.28 | 0.516 | 0.562 | 0.081 | 15 | 11.44 | 0.721 | ns | |
| SalE14 | 279 | 6 | 2.09 | 0.502 | 0.522 | 0.038 | 15 | 8.36 | 0.909 | ns | |
| SST-A6-I | 279 | 4 | 1.61 | 0.401 | 0.378 | −0.062 | 6 | 2.06 | 0.914 | ns | |
| SST-A6-II | 279 | 7 | 2.36 | 0.559 | 0.577 | 0.031 | 21 | 13.83 | 0.877 | ns | |
| SST-C2 | 279 | 13 | 2.94 | 0.631 | 0.659 | 0.043 | 78 | 81.94 | 0.358 | ns | |
| SST-C3 | 279 | 4 | 1.64 | 0.405 | 0.390 | −0.037 | 6 | 11.69 | 0.069 | ns | |
| SST-E11 | 279 | 13 | 4.70 | 0.749 | 0.787 | 0.048 | 78 | 75.77 | 0.550 | ns | |
| 2 | All loci | 44 | 5.23 (0.63) | 2.47 (0.33) | 0.533 (0.055) | 0.522 (0.054) | −0.025 (0.031) | 26 | 22.37 | 0.668 | ns |
| SalE2 | 44 | 7 | 2.54 | 0.682 | 0.607 | −0.123 | 21 | 12.30 | 0.931 | ns | |
| Sal3 | 44 | 5 | 2.71 | 0.591 | 0.631 | 0.064 | 10 | 27.40 | 0.002 | ns | |
| SalE5 | 44 | 1 | 1.00 | – | – | – | Monomorphic | ||||
| SalE6 | 44 | 4 | 1.87 | 0.386 | 0.465 | 0.168 | 6 | 7.20 | 0.302 | ns | |
| SalE7 | 44 | 6 | 2.22 | 0.523 | 0.550 | 0.050 | 15 | 12.57 | 0.635 | ns | |
| SalE8 | 44 | 6 | 4.50 | 0.750 | 0.778 | 0.036 | 15 | 12.42 | 0.647 | ns | |
| SalE11 | 44 | 4 | 2.07 | 0.591 | 0.517 | −0.142 | 6 | 6.72 | 0.347 | ns | |
| SalE14 | 44 | 3 | 1.96 | 0.500 | 0.491 | −0.019 | 3 | 1.27 | 0.737 | ns | |
| SST-A6-I | 44 | 4 | 1.75 | 0.500 | 0.429 | −0.165 | 6 | 5.89 | 0.436 | ns | |
| SST-A6-II | 44 | 7 | 2.21 | 0.636 | 0.547 | −0.163 | 21 | 6.19 | 0.999 | ns | |
| SST-C2 | 44 | 7 | 2.12 | 0.591 | 0.529 | −0.118 | 21 | 13.13 | 0.904 | ns | |
| SST-C3 | 44 | 4 | 1.77 | 0.409 | 0.435 | 0.059 | 6 | 1.63 | 0.951 | ns | |
| SST-E11 | 44 | 10 | 5.33 | 0.773 | 0.812 | 0.049 | 45 | 46.25 | 0.420 | ns | |
| (b) | |||||||||||
| 1 | All loci | 56 | 5.83 (0.44) | 2.47 (0.17) | 0.555 (0.036) | 0.574 (0.028) | 0.037 (0.030) | 24 | 20.35 | 0.677 | ns |
| SalE2 | 56 | 8 | 1.92 | 0.446 | 0.479 | 0.068 | 28 | 20.53 | 0.845 | ns | |
| Sal3 | 56 | 7 | 2.50 | 0.571 | 0.601 | 0.049 | 21 | 12.35 | 0.930 | ns | |
| SalE6 | 56 | 5 | 2.28 | 0.446 | 0.561 | 0.204 | 10 | 5.28 | 0.872 | ns | |
| SalE7 | 56 | 7 | 3.07 | 0.750 | 0.674 | −0.112 | 21 | 16.89 | 0.718 | ns | |
| SalE8 | 56 | 8 | 3.77 | 0.750 | 0.735 | −0.021 | 28 | 16.19 | 0.963 | ns | |
| SalE11 | 56 | 4 | 1.94 | 0.411 | 0.484 | 0.151 | 6 | 6.47 | 0.373 | ns | |
| SalE14 | 56 | 4 | 2.14 | 0.607 | 0.533 | −0.138 | 6 | 2.72 | 0.843 | ns | |
| Sal23 | 56 | 5 | 2.39 | 0.571 | 0.582 | 0.018 | 10 | 4.40 | 0.928 | ns | |
| SST-A6-II | 56 | 5 | 2.34 | 0.607 | 0.573 | −0.060 | 10 | 4.55 | 0.919 | ns | |
| SST-C2 | 56 | 7 | 3.11 | 0.625 | 0.679 | 0.079 | 21 | 9.02 | 0.989 | ns | |
| SST-C3 | 56 | 4 | 1.62 | 0.357 | 0.383 | 0.069 | 6 | 2.40 | 0.879 | ns | |
| SST-E11 | 56 | 6 | 2.52 | 0.518 | 0.604 | 0.143 | 15 | 14.55 | 0.484 | ns | |
| 2 | All loci | 3 | 2.83 (0.37) | 2.48 (0.31) | 0.639 (0.119) | 0.505 (0.075) | −0.242 (0.140) | – | – | – | – |
| SalE2 | 3 | 3 | 2.57 | 1.000 | 0.611 | −0.636 | 3 | 3.00 | 0.392 | ns | |
| Sal3 | 3 | 3 | 2.57 | 1.000 | 0.611 | −0.636 | 3 | 3.00 | 0.392 | ns | |
| SalE6 | 3 | 2 | 1.80 | 0.000 | 0.444 | 1.000 | 1 | 3.00 | 0.083 | ns | |
| SalE7 | 3 | 4 | 3.60 | 1.000 | 0.722 | −0.385 | 6 | 4.50 | 0.609 | ns | |
| SalE8 | 3 | 5 | 4.50 | 1.000 | 0.778 | −0.286 | 10 | 9.00 | 0.532 | ns | |
| SalE11 | 3 | 1 | 1.00 | – | – | – | Monomorphic | ||||
| SalE14 | 3 | 2 | 1.80 | 0.667 | 0.444 | −0.500 | 1 | 0.75 | 0.386 | ns | |
| Sal23 | 3 | 3 | 2.57 | 0.667 | 0.611 | −0.091 | 3 | 2.33 | 0.506 | ns | |
| SST-A6-II | 3 | 2 | 1.80 | 0.667 | 0.444 | −0.500 | 1 | 0.75 | 0.386 | ns | |
| SST-C2 | 3 | 4 | 3.00 | 0.667 | 0.667 | 0.000 | 6 | 6.33 | 0.387 | ns | |
| SST-C3 | 3 | 1 | 1.00 | – | – | – | Monomorphic | ||||
| SST-E11 | 3 | 4 | 3.60 | 1.000 | 0.722 | −0.385 | 6 | 4.50 | 0.609 | ns | |
| 3 | All loci | 54.83 (0.11) | 5.08 (0.43) | 2.59 (0.24) | 0.587 (0.027) | 0.590 (0.025) | 0.003 (0.030) | 24 | 24.60 | 0.428 | ns |
| SalE2 | 55 | 7 | 1.95 | 0.436 | 0.487 | 0.104 | 21 | 23.10 | 0.339 | ns | |
| Sal3 | 55 | 4 | 2.47 | 0.655 | 0.594 | −0.101 | 6 | 10.72 | 0.097 | ns | |
| SalE6 | 55 | 3 | 2.48 | 0.691 | 0.597 | −0.157 | 3 | 3.00 | 0.392 | ns | |
| SalE7 | 55 | 5 | 2.15 | 0.600 | 0.536 | −0.120 | 10 | 3.91 | 0.951 | ns | |
| SalE8 | 54 | 7 | 4.99 | 0.759 | 0.800 | 0.051 | 21 | 26.37 | 0.193 | ns | |
| SalE11 | 55 | 4 | 2.34 | 0.582 | 0.573 | −0.016 | 6 | 7.59 | 0.270 | ns | |
| SalE14 | 55 | 4 | 2.56 | 0.527 | 0.609 | 0.135 | 6 | 5.36 | 0.498 | ns | |
| Sal23 | 55 | 6 | 2.55 | 0.655 | 0.609 | −0.076 | 15 | 6.40 | 0.972 | ns | |
| SST-A6-II | 55 | 5 | 2.25 | 0.564 | 0.557 | −0.013 | 10 | 2.21 | 0.994 | ns | |
| SST-C2 | 54 | 7 | 3.24 | 0.593 | 0.691 | 0.142 | 21 | 9.77 | 0.982 | ns | |
| SST-C3 | 55 | 3 | 1.93 | 0.491 | 0.482 | −0.017 | 3 | 0.25 | 0.969 | ns | |
| SST-E11 | 55 | 6 | 2.21 | 0.491 | 0.548 | 0.104 | 15 | 15.16 | 0.440 | ns | |
| 4 | All loci | 11 | 3.75 (0.18) | 2.36 (0.14) | 0.591 (0.054) | 0.549 (0.044) | −0.074 (0.047) | 88 | 62.32 | 0.983 | ns |
| SalE2 | 11 | 4 | 2.55 | 0.727 | 0.607 | −0.197 | 6 | 3.97 | 0.680 | ns | |
| Sal3 | 11 | 4 | 2.81 | 0.727 | 0.645 | −0.128 | 6 | 2.58 | 0.859 | ns | |
| SalE6 | 11 | 3 | 2.28 | 0.545 | 0.562 | 0.029 | 3 | 1.83 | 0.608 | ns | |
| SalE7 | 11 | 4 | 2.55 | 0.636 | 0.607 | −0.048 | 6 | 3.20 | 0.783 | ns | |
| SalE8 | 11 | 4 | 2.44 | 0.545 | 0.591 | 0.077 | 6 | 11.55 | 0.073 | ns | |
| SalE11 | 11 | 4 | 2.30 | 0.455 | 0.566 | 0.197 | 6 | 3.28 | 0.772 | ns | |
| SalE14 | 11 | 4 | 2.92 | 0.818 | 0.657 | −0.245 | 6 | 4.27 | 0.640 | ns | |
| Sal23 | 11 | 4 | 2.20 | 0.636 | 0.545 | −0.167 | 6 | 1.67 | 0.947 | ns | |
| SST-A6-II | 11 | 4 | 2.18 | 0.727 | 0.541 | −0.344 | 6 | 3.59 | 0.732 | ns | |
| SST-C2 | 11 | 4 | 2.72 | 0.545 | 0.632 | 0.137 | 6 | 6.93 | 0.327 | ns | |
| SST-C3 | 11 | 2 | 1.10 | 0.091 | 0.087 | −0.048 | 1 | 0.02 | 0.875 | ns | |
| SST-E11 | 11 | 4 | 2.22 | 0.636 | 0.550 | −0.158 | 6 | 3.85 | 0.696 | ns | |
sig, significant; ns, not significant.