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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cytokine. 2015 Jan 23;72(1):71–85. doi: 10.1016/j.cyto.2014.11.007

Figure 2. OSM binds ECM proteins via electrostatic bonds: additional evidence for covalent disulfide bonds.

Figure 2

(A) Full-length 26 kDa OSM and mature 22 kDa OSM were bound to ECM in a 3 h incubation, 37°C in SFM at pH 5.5, 6.5 and 7.5. OSM was observed to bind to all ECM proteins in a pH-dependent fashion with greater 26 kDa OSM binding observed at pH 6.5–7.5. (B) The binding of full-length OSM compared to 24 kDa OSM. 24 kDa OSM was observed to bind to each ECM protein to a greater extent than 26 kDa OSM at pH 6.5–7.5, with more similar levels of binding apparent at pH 5.5. (C) The binding of OSM to ECM in the presence or absence of DTT (1 mM) inhibited, or partially inhibited the binding of OSM to each of the ECM proteins tested.