Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cytokine. 2015 Jan 23;72(1):71–85. doi: 10.1016/j.cyto.2014.11.007

Figure 4. OSM bound to ECM is biologically active.

Figure 4

STAT3 phosphorylation by OSM acting as a soluble ligand and as a solid-phase ligand immobilized to ECM was examined. (A) Time course examining the phosphorylation of STAT3 by soluble 26 kDa and 22 kDa OSM. (B) OSM immobilized to ECM proteins (2 h, 37°C, pH 7.5) induced pSTAT3 in T47D cells following a 2h incubation that was inhibited with a neutralizing antibody to OSM. (C) OSM immobilized to ECM (2 h, 37°C, pH 7.5) induced pSTAT3 in ZR-75-1 cells following a 24h incubation that was inhibited by a neutralizing antibody to OSM.