(a,b) Immunostaining of endogenous UNC-104 in wild-type (a) and unc-33(mn407) (b) animals, with corresponding schematic diagrams. Yellow arrowheads indicate UNC-104 immunoreactivity in sensory dendrite regions. nr, nerve ring; vnc, ventral nerve cord. (c,d) Localization of UNC-104::GFP in PVD neurons of wild-type (c) and unc-33 (mn407) (d) animals, with schematic diagrams. White arrowheads indicate UNC-104::GFP enrichment in PVD axons; yellow arrowheads indicate UNC-104::GFP in PVD dendrites.
(e) Quantification of animals with detectable UNC-104::GFP fluorescence in PVD dendrites (n>25 animals/genotype). Error bars indicate s.e.p.
(f,g) Inferred UNC-104/KIF1A activity in neurons of wild-type animals (f) and unc-33 mutants (g). Anterior is at left and dorsal is up in all panels. Scale bars, 10 μm.