Figure 2.
Analysis of genes involved in DNA damage response, cell cycle and survival signaling in A2780 and A2780cis tumors after carboplatin short-term treatment. (A) RNA expression analysis of DNA damage response and NF-κB signaling assessed from Illumina bead chip array. (B) Bcl-2, cyclin D1 and p21Waf1/Cip1 protein levels. Tumors (n = 3) were pooled and subjected to western blot analysis. (C) Protein phosphorylation levels determined by Proteome Profiler Array. Significant differential protein phosphorylation was determined by comparison of all groups with Two-Way-ANOVA (P < 0.001). (D) Validation of Proteome Profiler Array data using phospho/total AKT western blot analysis.