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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 30;95(4):660–677. doi: 10.1111/mmi.12893

FIG. 2. Deletion of the entire prgA-C gene cluster only modestly diminishes plasmid transfer.

FIG. 2

(A) Transfer frequencies of OG1RF strains lacking or carrying pCF10, pCF10ΔprgA-C alone or with the PQ::prgA-C expression plasmid pINY1801 in 2 h filter and liquid matings. Transfer frequencies are presented as the number of transconjugants per donor cell (Tc's/Donor). Experiments were repeated at least 3 times and the histogram depicts average values with standard deviations. (B) qRT-PCR results showing the relative expression levels of regions of the prgQ operon located upstream (QL) and downstream (prgD, prgJ, pcfC, pcfG) of the prgA-C genes in pCF10 (solid lines) and pCF10ΔprgA-C (dashed lines) at 30 and 60 min following cCF10 pheromone induction with 5ng/ml cCF10. The data shown are from one biological replicate, which was repeated with similar results. (C) Steady state levels of pCF10-encoded surface proteins (PrgA, PrgB, PrgC) and downstream T4SS machine subunits (PrgJ, PcfC, PcfG) in E. faecalis OG1RF without (−) and with pCF10; pCF10ΔprgA-C or pCF10ΔprgA-C and the PQ::prgA-C expression plasmid pINY1801. Immunoblots were developed with antibodies to the proteins listed on the left. Protein extracts were loaded on a per-cell equivalent basis.