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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 30;95(4):660–677. doi: 10.1111/mmi.12893

FIG. 3. PrgB-mediated aggregation and plasmid transfer is dependent on eDNA.

FIG. 3

(A) Aggregation assays showing the effects of DNase treatment on pheromone (cCF10)-induced cellular aggregation. DNase treatment inhibited aggregation of OG1RF(pCF10) (upper graph) and OG1RF(pCF10ΔprgB, pMB6) expressing PQ::prgB (lower graph). (B) Photographs of cells grown in microtiter plates showing the effects of DNase on pheromone-induced clumping at 14 h following pheromone induction. Strains: OG1RF lacking or carrying pCF10, pCF10ΔprgB, or pCF10ΔprgB and pMB6. The numbers correspond to colony forming units (CFU's) per ml at 14 h following cCF10 induction in absence (Untreated) or presence of DNase. (C) Histograms depict the transfer frequencies of pCF10, pCF10ΔprgB and pCF10ΔprgA-C without or with DNase added at the onset of a 2 h liquid mating.