Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 30;95(4):660–677. doi: 10.1111/mmi.12893

FIG. 5. PrgA and PrgB mediate development of robust, mature biofilms on PMMA coupons.

FIG. 5

Macroscopic images of biofilms produced by strains inoculated on PMMA coupons at the onset of cCF10 pheromone induction and incubated for 48 h in TSB medium. Cells and eDNA were stained with hexidium iodide (red), extracellular polysaccharide (EPS) was stained with calcofluor white (green). Merged two-color images and corresponding separated color channels of representative 48-h biofilms at 40× magnification are shown; scale bars, 50 μm. Sagittal views are shown depicting biofilm thicknesses with measured values shown in μM; scale bars, 3.125 μm. Strains: OG1RF lacking or carrying pCF10 or pINY1801 (PQ::prgA-C) or the pCF10 variants ΔprgA-C, ΔprgA, ΔprgB, or ΔprgC.