TABLE 1.
Plasmids used in this study.
| Plasmid | Relevant features | Source or Reference |
|---|---|---|
| pCF10 | Tetr, pheromone inducible conjugative plasmid | (Dunny et al, 1981) |
| pCF10ΔoriT | Tetr, pCF10 with a 54-bp deletion within oriT | (Staddon et al., 2006) |
| pCF10ΔprgA | Tetr, pCF10 deleted of prgA | This study |
| pCF10ΔoriTΔprgA | Tetr, pCF10 deleted of oriT and prgA | This study |
| pCF10-8, here designated pCF10ΔprgB | Tetr, pCF10 deleted of prgB | (Chuang-Smith et al., 2010) |
| pCF10ΔprgC | Tetr, pCF10 deleted of prgC | This study |
| pCF10ΔoriTΔprgC | Tetr, pCF10 deleted of oriT and prgC | This study |
| pCF10ΔprgA-C | Tetr, pCF10 deleted of prgA, prgB, prgU and prgC | This study |
| pDLP278p23 | Spcr, pDL278 with L. lactis constitutive promoter P23 | (Chen et al., 2007) |
| pMB2 | Spcr, pDL278p23 expressing P23::prgA | This study |
| pMB3 | Spcr, pDL278p23 expressing P23::prgB | This study |
| pMB4 | Spcr, pDL278p23 expressing P23::prgC | This study |
| pCIE | Cmr, cCF10 inducible PQ expression vector | Dunny Lab |
| pMB5 | Cmr, pCIE expressing Pq::prgA | This study |
| pMB6 | Cmr, pCIE expressing PQ::prgB | This study |
| pMB7 | Cmr, pCIE expressing PQ::prgC | This study |
| pINY1801 | Cmr, fragment of pCF10 cloned in shuttle vector pWM401; expresses Pq::prgA-C | (Christie et al., 1988) |