(A, B) Enzyme Immmunoassay-EIA showed the presence of COX generated PGE2 and free-radical generated 8-isoprostanes in the PF. The levels of PGE2 (p<0.07) and 8-isoprostanes (p<0.005) were higher in the PF from endometriosis patients (n=43) compared to control women (n=36). Results are expressed as pg/ml ± SEM. (C) LC-MS/MS separation of PF lipids showed the presence of higher levels of both COX generated prostanoids (PGE2, PGD2) as well as 12, 15, 5-LOX generated 12, 15, 5-HETEs in the PF from endometriosis (n=6) patients compared to control women (n=6). T-test was used for statistical analysis. P<0.05 was considered significant.