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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Pain. 2015 Mar;156(3):528–539. doi: 10.1097/01.j.pain.0000460321.72396.88

Figure 2. Detection of prostaglandin-like molecules by LC-MS/MS.

Figure 2

(A) Oxidatively-modified LDL preparations were used to detect the presence of prostaglandin-like molecules using PGE2 EIA kit. The levels of prostaglandin-like molecules as measured by EIA increased as the level of oxidation of LDL increased, with L0 (control group) having the least level compared to L3 (n=10-12). (B and C) LC-MS/MS was used to detect eicosanoids generated by non-enzymatic oxidation of LDLs. Both enzymatic (PGE2, PGD2), and non-enzymatically generated oxidation products were generated during the oxidation of lipoproteins. One-way ANOVA followed by Dunnetts’ multiple comparison test was used for statistical analysis. P<0.05 was considered significant.