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. Author manuscript; available in PMC: 2015 Nov 18.
Published in final edited form as: Neuroendocrinology. 2014 Nov 18;100(4):334–346. doi: 10.1159/000369820

Figure 6.

Figure 6

Representative micrographs of GnRH and GPER immunolabeling in the ME. A low magnification image of GnRH-IR in a coronal section through the ME is shown (A) with boxes around the regions of the internal (left) and external (right) zones that were analyzed. The third ventricle (3V) is labeled for orientation. High magnification micrographs of punctate GnRH-IR (red) and GPER-IR (green) are shown in the internal (B) and external (C) zones of the ME. Colocalized puncta (yellow) are indicated with arrowheads. The insets show a high magnification example of colocalization in each region. Scale bars = 350 µm (A), 20 µm (B & C), 5 µm (insets).