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. Author manuscript; available in PMC: 2016 Feb 8.
Published in final edited form as: Breast Cancer Res Treat. 2015 Feb 8;149(3):681–692. doi: 10.1007/s10549-015-3279-5

Fig. 3.

Fig. 3

Effect of Bmi-1 RNA interference on cell migration inhibition by BITC. a Western blotting for Bmi-1 in MDA-MB-231 cells transiently transfected with control siRNA or Bmi-1-targeted siRNA. b Representative migration images (Boyden chamber assay) for MDA-MB-231 cells transfected with control siRNA or Bmi-1-targeted siRNA after 24 h treatment with DMSO or 2.5 μM BITC (100× magnification). c Quantitation of cell migration in MDA-MB-231 cells. d Western blotting for Bmi-1 in SUM159 cells transiently transfected with control siRNA or Bmi-1 targeted siRNA. e Quantitation of cell migration for SUM159 cells. Results in panel c and e are mean ± SD; n=3. *Significantly different (P<0.05) between the indicated groups by one-way ANOVA followed by Bonferroni’s multiple comparison test.