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. Author manuscript; available in PMC: 2016 Feb 13.
Published in final edited form as: Circ Res. 2015 Jan 6;116(4):600–611. doi: 10.1161/CIRCRESAHA.116.304899

Figure 1. Increased receptor interacting protein 3 (RIP3) expression and cell necrosis in abdominal aortic aneurysm tissues.

Figure 1

(A) Representative photographs of human aneurysmal and normal aortic cross sections that were double stained for RIP3 (or RIP1, red) and smooth muscle α-Actin (SM-αA, green). n=4. Scale bars=50μm. (B) Real-time PCR (n=8) and Western blotting (n=4) analyses of Rip3 expression in C57BL/6 mouse arteries perfused with elastase or heat-inactivated elastase on Day 14 post-perfusion. Data are mean±SEM. (C) Representative photographs of aortic cross-sections harvested from C57BL/6 mice on Day 3 post-surgery. Sections were co-stained for RIP3 and TUNEL. Higher magnified views of highlighted regions were shown on the right. L indicates lumen. n=3. Scale bars=50μm. (D) Representative photographs of aortic cross sections of normal or elastase-perfused aortae (Day 7 post-perfusion). Propidium iodide (PI) was administered to mice via intraperitoneal injection 2 hours prior to sacrifice. Following PI staining, aortic sections were also stained for CD31 or SM-αA. n=3. Scale bars=100μm.